[Shyi-Dong Yeh] Modification of the helper-component protease of papaya ringspot virus Vietnam isolate to generate attenuated mutants for disease management by cross protection
An infectious cDNA clone was constructed from PRSV isolate TG5 from South Vietnam for generation of mild mutants with point mutations on the essential motifs of HC-Pro by site-directed mutagenesis. Mutants TG-d5, TG-d5I7, and TG-d5L206 containing d5, d5 + F7I, and d5 + F206L were accumulated in papaya at reduced levels with a zigzag pattern and stable beyond six monthly passages. Our results indicate that the protection by the three mutants are suitable for the control of PRSV in South Vietnam.
Tran, et al., 2022 Phytopathology
Article source: https://doi.org/10.1094/PHYTO-05-22-0168-R